The explants were grown on various combinations of auxins iaa, iba, naa, 2,4d and cytokinins k, ba to induce callus and regeneration of organs. Qualitative and quantitative changes in putrescine, spermidine, and spermine tissue contents, during the rooting process and their correlation with other biochemical markers and morphological changes, provided the rationale for their use during the study of a. Como hacer enraizante natural con lentejas ecocosas. Conference paper pdf available january 2010 with 152 reads. In vitro propagation of solanum dolichosepalum solanaceae. The aim of the study was to evaluate the in vitro propagation ability of 10 clonal lines of thorny globe artichoke cynara scolymus. During the rooting testing process, the culture medium used was the one formulated by.
Establishment of callus tissue culture from cotyledon fragments of castanea sativa mill. In vitro shoot cultures from nodal explants of mature trees were established and multiplied as reported by, 14, 15. In this study we used three different auxins at various concentrations 0, 0. Changes in photosynthetic metabolism during in vitro growth and subsequent acclimatisation of vitroplants. The shoots were separated and placed in another ms 1962 medium supplemented with indolebutyric acid iba. In vitro propagation of some olive olea europaea sativa l. How to make homemade hormone rooting powder or tonic. The study methodology comprised five stages of evaluation.
Scientia horticulturae, 24 1984 1234 123 elsevier science publishers b. Micropropagation of clonal lines of thorny artichoke cynara. Callo basal, enraizamiento, lulo, micropropagacion, solanaceas silvestres. Scribd is the worlds largest social reading and publishing site. Efficient in vitro rooting of chrysanthemum morifolium ramat. Cultivares, cultivo in vitro, micropropagacion, pina, propagacion masiva. Jan 30, 2020 how to make homemade hormone rooting powder or tonic. Pdf caracterizacion fisiologica del enraizamiento in. Seeds of 10 families of gmelina arborea gmelina obtained from controlled crosses, were established in vitro. Pdf en espanol articulo en xml referencias del articulo como citar. Micropropagation of clones from controlled crosses of gmelina.
Influence of auxin concentration on in vitro rooting of. Nodal segments of seedlings were placed in murashige and skoog ms 1962 basal medium supplemented with different concentrations of benzyladenine ba. Nodal segments of seedlings were placed in murashige and skoog ms 1962. The stages evaluated were initiation, multiplication, rooting, acclimatization, and transplant to the field. Cuerda correa 1, maria teresa marin bosca 2, cristobal valenzuela calahorro 3. Micropropagated sugarcane seedlings were used by tissue culture. Like several other woody species, walnut tree explants obtained from embryonic tissues are easily established in vitro and multiplied by direct organogenesis.
However, in vitro propagation through seedling has limitation in tree improvement programme. In vitro culture of cotyledon tissue of castanea sativa mill. Rooting hormones help plant cuttings grow better and form strong root systems. The research was carried out in the greenhouse of the faculty of agronomy of the autonomous university of sinaloa, located at 243729n and 1072636w, in sinaloa, mexico. While you can buy many rooting powders or tonics from gardening stores, you can make your own using things. Dose of paclobutrazol in the growth of sugarcane seedlings in. Establishment and in vitro clonal propagation of the spanish.
554 321 224 555 672 300 636 107 1352 1358 1075 484 184 1104 74 1080 1209 1338 34 1331 681 690 512 20 1134 1305 956 493 1318 124 675 830 4 322 933 1132 337 764 455 237 92 603 1416 168